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Abstract Human induced pluripotent stem cells (iPSCs) hold great promise for reducing the mortality of cardiovascular disease by cellular replacement of infarcted cardiomyocytes (CMs). CM differentiation via iPSCs is a lengthy multiweek process and is highly subject to batch‐to‐batch variability, presenting challenges in current cell manufacturing contexts. Real‐time, label‐free control quality attributes (CQAs) are required to ensure efficient iPSC‐derived CM manufacturing. In this work, we report that live oxygen consumption rate measurements are highly predictive CQAs of CM differentiation outcome as early as the first 72 h of the differentiation protocol with an accuracy of 93%. Oxygen probes are already incorporated in commercial bioreactors, thus methods presented in this work are easily translatable to the manufacturing setting. Detecting deviations in the CM differentiation trajectory early in the protocol will save time and money for both manufacturers and patients, bringing iPSC‐derived CM one step closer to clinical use. 

Biodiversity loss poses a major threat to ecosystem function, which has already been severely impacted by global late-Quaternary defaunation. The loss of mammalian megafauna from many insular systems has rendered reptiles into key modulators of many ecosystem services, such as seed dispersal and pollination. How late-Quaternary extinction events impacted reptile functional diversity remains unclear but can provide critical guidance on traits that render reptiles vulnerable to extinction, as well as anthropogenic, environmental, and evolutionary histories that may promote stability and resilience. This study reconstructs the trajectory of functional diversity change in the Caribbean reptile fauna, a speciose biota distributed over a diverse set of islands with heterogeneous histories of human habitation and exploitation. Human-induced Quaternary extinctions have completely removed key functional entities (FEs)—groupings of species with similar traits that are expected to provide similar ecosystem services—from the region, but functional redundancy on large islands served as a buffer to major functional diversity loss. Small islands, on the other hand, lose up to 67% of their native FEs with only a few exceptions, underscoring the importance of a place’s anthropogenic history in shaping present-day biodiversity. While functional redundancy has shielded ecosystems from significant functional diversity loss in the past, it is being eroded and not replenished by species introductions, leaving many native FEs and the communities that they support vulnerable to extinction and functional collapse. This research provides critical data on long-term functional diversity loss for a taxonomic group whose contributions to ecosystem function are understudied and undervalued. 

Terrestrial ecosystems encompass a vast and vital component of Earth's biodiversity and ecosystem services. The effect of increased anthropogenic dominance on terrestrial communities defines major challenges for ecosystem conservation, including habitat destruction and fragmentation, climate change, species invasions and extinctions, and disease spread. Here, we integrate fossil, historical, and present-day organismal and ecological data to investigate how conservation paleobiology provides deep-time perspectives on terrestrial organisms, populations, communities, and ecosystems impacted by anthropogenic processes. We relate research tools to conservation outputs and highlight gaps that currently limit conservation paleobiology from reaching its full impact on conservation practice and management. In doing so, we also highlight how the colonial legacies of conservation biology and paleobiology confound our understanding of present-day biodiversity, ecosystem processes, and conservation outlooks, and we make recommendations for more inclusive and ethical practices moving forward. 

The ability to engineer complex multicellular systems has enormous potential to inform our understanding of biological processes and disease and alter the drug development process. Engineering living systems to emulate natural processes or to incorporate new functions relies on a detailed understanding of the biochemical, mechanical, and other cues between cells and between cells and their environment that result in the coordinated action of multicellular systems. On April 3–6, 2022, experts in the field met at the Keystone symposium “Engineering Multicellular Living Systems” to discuss recent advances in understanding how cells cooperate within a multicellular system, as well as recent efforts to engineer systems like organ-on-a-chip models, biological robots, and organoids. Given the similarities and common themes, this meeting was held in conjunction with the symposium “Organoids as Tools for Fundamental Discovery and Translation”.